High zinc concentrations reduce rooting capacity and alter metallothionein gene expression in white poplar (Populus alba L. cv. Villafranca).
Identifieur interne : 003B62 ( Main/Exploration ); précédent : 003B61; suivant : 003B63High zinc concentrations reduce rooting capacity and alter metallothionein gene expression in white poplar (Populus alba L. cv. Villafranca).
Auteurs : Stefano Castiglione [Italie] ; Cinzia Franchin ; Tiziana Fossati ; Guido Lingua ; Patrizia Torrigiani ; Stefania BiondiSource :
- Chemosphere [ 0045-6535 ] ; 2007.
Descripteurs français
- KwdFr :
- Chlorophylle (métabolisme), Clonage d'organisme (MeSH), Dépollution biologique de l'environnement (MeSH), Feuilles de plante (métabolisme), Métallothionéine (biosynthèse), Métallothionéine (génétique), Populus (effets des médicaments et des substances chimiques), Pousses de plante (croissance et développement), Pousses de plante (effets des médicaments et des substances chimiques), Racines de plante (croissance et développement), Racines de plante (effets des médicaments et des substances chimiques), Racines de plante (métabolisme), Régulation de l'expression des gènes (effets des médicaments et des substances chimiques), Tiges de plante (métabolisme), Zinc (métabolisme), Zinc (toxicité).
- MESH :
- biosynthèse : Métallothionéine.
- croissance et développement : Pousses de plante, Racines de plante.
- effets des médicaments et des substances chimiques : Populus, Pousses de plante, Racines de plante, Régulation de l'expression des gènes.
- génétique : Métallothionéine.
- métabolisme : Chlorophylle, Feuilles de plante, Racines de plante, Tiges de plante, Zinc.
- toxicité : Zinc.
- Clonage d'organisme, Dépollution biologique de l'environnement.
English descriptors
- KwdEn :
- Biodegradation, Environmental (MeSH), Chlorophyll (metabolism), Cloning, Organism (MeSH), Gene Expression Regulation (drug effects), Metallothionein (biosynthesis), Metallothionein (genetics), Plant Leaves (metabolism), Plant Roots (drug effects), Plant Roots (growth & development), Plant Roots (metabolism), Plant Shoots (drug effects), Plant Shoots (growth & development), Plant Stems (metabolism), Populus (drug effects), Zinc (metabolism), Zinc (toxicity).
- MESH :
- chemical , biosynthesis : Metallothionein.
- chemical , genetics : Metallothionein.
- chemical , metabolism : Chlorophyll, Zinc.
- drug effects : Gene Expression Regulation, Plant Roots, Plant Shoots, Populus.
- growth & development : Plant Roots, Plant Shoots.
- metabolism : Plant Leaves, Plant Roots, Plant Stems.
- chemical , toxicity : Zinc.
- Biodegradation, Environmental, Cloning, Organism.
Abstract
Poplar is a good candidate for phytoremediation purposes because of its rapid growth, extensive root system, and ease of propagation and transformation; however its tolerance to heavy metals has not been fully investigated yet. In the present work, an in vitro model system with shoot cultures was used to investigate the tolerance to high concentrations of zinc (Zn) of a commercial clone (Villafranca) of Populus alba. Based on chlorophyll content (leaf chlorosis) and the rate of adventitious root formation from shoot cuttings as parameters of damage, 0.5-4mM zinc concentrations were all toxic albeit to different extents. Northern blot and reverse transcriptase (RT)-PCR analyses were used to examine the expression profiles of types 1, 2 and 3 PaMT genes in stems, leaves and roots of plants exposed to Zn treatments. In leaves, MT1 and MT3 mRNA levels were enhanced by Zn, while MT2 transcripts were not affected. The PaMT expression profiles were differentially affected by Zn in an organ-specific manner, and the relationship with Zn concentration and exposure time was rarely linear. The developmental and molecular data reveal that the in vitro model is a sensitive and reliable system to study heavy metal stress responses.
DOI: 10.1016/j.chemosphere.2006.11.039
PubMed: 17223164
Affiliations:
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Le document en format XML
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uniqKey="Torrigiani P" first="Patrizia" last="Torrigiani">Patrizia Torrigiani</name></author><author><name sortKey="Biondi, Stefania" sort="Biondi, Stefania" uniqKey="Biondi S" first="Stefania" last="Biondi">Stefania Biondi</name></author></analytic><series><title level="j">Chemosphere</title><idno type="ISSN">0045-6535</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biodegradation, Environmental (MeSH)</term><term>Chlorophyll (metabolism)</term><term>Cloning, Organism (MeSH)</term><term>Gene Expression Regulation (drug effects)</term><term>Metallothionein (biosynthesis)</term><term>Metallothionein (genetics)</term><term>Plant Leaves (metabolism)</term><term>Plant Roots (drug effects)</term><term>Plant Roots (growth & development)</term><term>Plant Roots (metabolism)</term><term>Plant Shoots (drug effects)</term><term>Plant Shoots (growth & 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In the present work, an in vitro model system with shoot cultures was used to investigate the tolerance to high concentrations of zinc (Zn) of a commercial clone (Villafranca) of Populus alba. Based on chlorophyll content (leaf chlorosis) and the rate of adventitious root formation from shoot cuttings as parameters of damage, 0.5-4mM zinc concentrations were all toxic albeit to different extents. Northern blot and reverse transcriptase (RT)-PCR analyses were used to examine the expression profiles of types 1, 2 and 3 PaMT genes in stems, leaves and roots of plants exposed to Zn treatments. In leaves, MT1 and MT3 mRNA levels were enhanced by Zn, while MT2 transcripts were not affected. The PaMT expression profiles were differentially affected by Zn in an organ-specific manner, and the relationship with Zn concentration and exposure time was rarely linear. The developmental and molecular data reveal that the in vitro model is a sensitive and reliable system to study heavy metal stress responses.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17223164</PMID><DateCompleted><Year>2007</Year><Month>06</Month><Day>19</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0045-6535</ISSN><JournalIssue CitedMedium="Print"><Volume>67</Volume><Issue>6</Issue><PubDate><Year>2007</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Chemosphere</Title><ISOAbbreviation>Chemosphere</ISOAbbreviation></Journal><ArticleTitle>High zinc concentrations reduce rooting capacity and alter metallothionein gene expression in white poplar (Populus alba L. cv. Villafranca).</ArticleTitle><Pagination><MedlinePgn>1117-26</MedlinePgn></Pagination><Abstract><AbstractText>Poplar is a good candidate for phytoremediation purposes because of its rapid growth, extensive root system, and ease of propagation and transformation; however its tolerance to heavy metals has not been fully investigated yet. In the present work, an in vitro model system with shoot cultures was used to investigate the tolerance to high concentrations of zinc (Zn) of a commercial clone (Villafranca) of Populus alba. Based on chlorophyll content (leaf chlorosis) and the rate of adventitious root formation from shoot cuttings as parameters of damage, 0.5-4mM zinc concentrations were all toxic albeit to different extents. Northern blot and reverse transcriptase (RT)-PCR analyses were used to examine the expression profiles of types 1, 2 and 3 PaMT genes in stems, leaves and roots of plants exposed to Zn treatments. In leaves, MT1 and MT3 mRNA levels were enhanced by Zn, while MT2 transcripts were not affected. The PaMT expression profiles were differentially affected by Zn in an organ-specific manner, and the relationship with Zn concentration and exposure time was rarely linear. The developmental and molecular data reveal that the in vitro model is a sensitive and reliable system to study heavy metal stress responses.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Castiglione</LastName><ForeName>Stefano</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Dipartimento di Biologia, Università di Milano, Milano, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Franchin</LastName><ForeName>Cinzia</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Fossati</LastName><ForeName>Tiziana</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Lingua</LastName><ForeName>Guido</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Torrigiani</LastName><ForeName>Patrizia</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Biondi</LastName><ForeName>Stefania</ForeName><Initials>S</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2007</Year><Month>01</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Chemosphere</MedlineTA><NlmUniqueID>0320657</NlmUniqueID><ISSNLinking>0045-6535</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>1406-65-1</RegistryNumber><NameOfSubstance UI="D002734">Chlorophyll</NameOfSubstance></Chemical><Chemical><RegistryNumber>9038-94-2</RegistryNumber><NameOfSubstance UI="D008668">Metallothionein</NameOfSubstance></Chemical><Chemical><RegistryNumber>J41CSQ7QDS</RegistryNumber><NameOfSubstance UI="D015032">Zinc</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="N">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002734" MajorTopicYN="N">Chlorophyll</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019976" MajorTopicYN="N">Cloning, Organism</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005786" MajorTopicYN="N">Gene Expression Regulation</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008668" MajorTopicYN="N">Metallothionein</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018520" MajorTopicYN="N">Plant Shoots</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018547" MajorTopicYN="N">Plant Stems</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015032" MajorTopicYN="N">Zinc</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000633" MajorTopicYN="Y">toxicity</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2006</Year><Month>05</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2006</Year><Month>11</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2006</Year><Month>11</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2007</Year><Month>1</Month><Day>16</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>6</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2007</Year><Month>1</Month><Day>16</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17223164</ArticleId><ArticleId IdType="pii">S0045-6535(06)01632-8</ArticleId><ArticleId IdType="doi">10.1016/j.chemosphere.2006.11.039</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Italie</li></country></list><tree><noCountry><name sortKey="Biondi, Stefania" sort="Biondi, Stefania" uniqKey="Biondi S" first="Stefania" last="Biondi">Stefania Biondi</name><name sortKey="Fossati, Tiziana" sort="Fossati, Tiziana" uniqKey="Fossati T" first="Tiziana" last="Fossati">Tiziana Fossati</name><name sortKey="Franchin, Cinzia" sort="Franchin, Cinzia" uniqKey="Franchin C" first="Cinzia" last="Franchin">Cinzia Franchin</name><name sortKey="Lingua, Guido" sort="Lingua, Guido" uniqKey="Lingua G" first="Guido" last="Lingua">Guido Lingua</name><name sortKey="Torrigiani, Patrizia" sort="Torrigiani, Patrizia" uniqKey="Torrigiani P" first="Patrizia" last="Torrigiani">Patrizia Torrigiani</name></noCountry><country name="Italie"><noRegion><name sortKey="Castiglione, Stefano" sort="Castiglione, Stefano" uniqKey="Castiglione S" first="Stefano" last="Castiglione">Stefano Castiglione</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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